A branch of botany is
1. Genetic engineering
2. Plant Tissue culture
3. Plant Physiology
4. Plant ecology
An example of a fusogenic agent is
1.Methanol
2. Ethyl alcohol
3. Polyethelene alcohol
4. Polyethelene glycol (PEG)
Bacillus subtilis and streptomyces spp. are Prokaryotes which are used as hosts for introducing
1.Plasmid
2.Replicon
3.Auxin
4.rDNA
Cells of higher plants are grown artificially in laboratory conditions using the technique of
1. Molecular cloning
2. Gene cloning
3. Tissue culture
4. Hybridzation
Cellulase and Cellulysin are the cell wall degrading enzyme mixtures which can digest
1. Cell wall
2. Epidermal cells
3. Mesophyll
4. Xylem vessels
DNA ligase was discovered in
1.1906
2.1910
3.1966
4.1970
DNA of donor is cut into pieces using
1. Vector
2. Plasmid
3. Endonuclease
4. Inoculum
DNA recombinant technology is also known as
1.Gene manipulation
2.Totipotency
3.Splicing
4.Gene cloning
Each colony is a collection of bacteria having many copies of rDNA fragments called
1. Molecular cloning
2.Splicing
3.Cloning vehicles
4.Vectors
Flamed and cooled platinum wire is used to transfer
1.Bacteria
2.Fungi
3.Callus
4.Inoculum
Foot and mouth disease of cattle is cured by
1. Insulin
2.Cytokinin
3. Vaccine
4.Interferon
Genes are directly introduced into the eukaryotie host for cloning through a
1.Plasmid
2. Vector
3. Endonuclease
4. Hormone
Genetic engineering started with flying colours in the early
1.1900
2.1950
3.1960
4.1970
If the DNA fragments of donor do not replicate in the host they are attached to a suitable
1. DNA ligase
2.Plasmid
3.Splicing
4.Replicon
Industrial production of enzymes vitamins vaccines and bio-fertilizers are the products of
1.Ploidy
2.Genetics
3.Physiology
4.Biotechnology
Inoculated culture vessels are protected from contamination of other organism by
1.Cleaning
2. Cotton plug
3. Oil cloth
4. Cork plug
Inter specific crosses in industrial micro-organisms are made easier by
1. Protoplast fusion
2. Tissue culture
3. Genetic engineering
4.Totipotency
Introducing the nitrogen fixing genes from bacteria to major food crops is possible through
1. Tissue culture
2.Hybridization
3. Protoplast
4. Genetic engineering
Joining of donor DNA fragments and vector DNA fragments with the help of DNA ligase enzyme is known as
1. Gene cloning
2.Splicing
3.Gene manipulation
4.Molecular cloning
Molecular cloning is also known as
1. Splicing
2.Gene cloning
3.Gene manipulation
4.Hardening
Plants obtained through tissue culture method constitute genetically uniform population called
1. Clone
2. Callus
3.Inoculum
4.Hardening
Protoplasmic fusion is of great importance in plants which are incompatible to
1. Molecular cloning
2. Gene cloning
3.Hybridization
4. Tissue culture
Replicons are also known as
1.Ligase
2.Plasmids
3.Auxin
4.Vectors
Restriction endonucleases are discovered in
1.1910
2.1906
3.1966
4.1970
Several plantlets are developed from the
1. Clone
2.Inoculum
3.Callus
4.Plasmid
Somatic hybridization is brought about by employing the technique of
1.Tissue culture
2.Splicing
3.Totipotency
4.Hardening
Suitable host in genetic engineering to introduce DNA fragments of donor is
1.Yeast
2. Bacillus subtilis
3. Escherichia coli
4. Bacteriophages
The ability of every living plant cell to produce the entire plant is called
1. Plasmid
2. Protoplast
3. Gene cloning
4. Totipotency
The agent which promotes the fusion of protoplasts is called
1. Mutagenic agent
2. Fusogenic agent
3. Chemical agent
4. Cellulase
The antiviral protein produced by virus infected cells is called
1.Insulin
2. Auxin
3. Vaccine
4. Interferon
The cells in tissue culture divide and form a mass of undifferentiated tissue called
1.Inoculum
2. Plasmid
3. Replicon
4. Callus
The contaminated microorganism in culture medium is
1.Fungi
2.Algae
3.Callus
4.Cytokinin
The culture medium is prepared from
1. Proteins
2.Vitamins
3. Agar
4.Bacteria
The DNA of vector is cut into fragments by
1.plasmid
2. Auxin
3. Restriction endonucleases
4. Cytokinin
The donor DNA fragments and vector DNA fragments are joined together by
1.DNA ligase
2.Plasmid
3.Auxin
4.Cytokinin
The enzyme which acts like a paste molecule to join DNA fragments
1.Plasmid
2.Clone
3. DNA ligase
4.Protease
The extra-chromosomal circular DNA found in the E.coli is
1.Plasmid
2. DNA ligase
3.Vector
4. Plasmid
The first step in protoplasmic fusion is the
1. Isolation of protoplasts
2. Fusion of protoplasts
3. Growth protoplasts
4. Regeneration of cell wall
The following is not a branch of biotechnology
1.Genetic engineering
2.Tissue culture
3.Physiology
4.Protoplast fusion
The following is used in industrial fermentation to produce food and drinks
1.Yeast
2.Algae
3.Vitamins
4.Vaccines
The following ratio of hormonal concentration is required to induce callus to form shoot and root
1.Gibberellins to auxin
2. Auxin to cytokinin
3. Gibberellins to cytokinin
4. Ethylene to auxins
The host bacterium follows the instruction of the 2
1.DNA ligase
2.Foreign rDNA
3.Plasmid
4.Auxin
The inherent capacity of an isolated plant cell to give rise to a whole plant is called
1. Totipotency
2. Protoplast
3. Hybridization
4. Gene manipulation
The instrument used for keeping inoculated vessels at a desired constant temperature is
1. Platinum wire
2.Micropipette
3. Petriplate
4. Incubator
The instrument used to transfer inoculums under aseptic condition is
1.Incubator
2. Culture tube
3. Micropipette
4. Petriplate
The interspecfic somatic hybrids of tobacco and petunia are produced by the technique of
1. Tissue culture
2. Protoplasmic fusion
3. Genetic engineering
4. Gene cloning
The isolated protoplasts are grown in
1.Hypotonic culture medium
2. Hypertonic culture medium
3. Isotonic culture medium
4. Agar culture medium
The isolated protoplasts are made to fuse to produce a hybrid cell in
1. Tissue culture
2. Genetic engineering
3.Protoplast fusion
4. Gene cloning
The mechanism which has the ability to engineer new organisms is known as
1.Totipotency
2.Molecular cloning
3.Genetic engineering
4.Splicing
The molecular scissors which cut DNA at specific sites are
1. Plasmids
2. Fusogenic agents
3.Inoculum
4. Restriction enzymes
The most suitable vectors are
1.Yeast
2.Bacteriophages
3.Moulds
4.Algae
The other name for hybrid DNA is
1.Recombinant DNA (rDNA)
2. DNA ligase
3.Plasmid
4.Replicon
The plant cell walls are digested by specific cell wall degrading
1. Protein mixtures
2. Vitamin mixtures
3.Enzyme mixtures
4. Hormonal mixtures
The process of transferring plantlets to pots and subjected gradually to natural environmental conditions is called
1. Hardening
2.Totipotency
3.Cloning
4.Splicing
The production of insulin interferon human growth hormones proteins and vaccines are based on
1. Tissue culture
2. Protoplast fusion
3. Genetic engineering
4. Hybridization
The production of new characteristics by introducing new genes and altering the genome is known as
1.DNA recombinant technology
2. Protoplast fusion
3.Totipotency
4.Splicing
The rapid and cheaper method of propagating plants with desire qualities is
1. Molecular cloning
2. Gene cloning
3. Tissue cloning
4. Genetic cloning
The rDNA is introduced into this host
1. E.coli
2.Yeast
3.Mould
4.Algae
The recent and fascinating branch of biology is
1. Physiology
2.Genetics
3. Genetic engineering
4.Ecology
The rigid plant cell wall is digested by
1.Pectinase
2. Protease
3. Amylase
4.Sucrase
The technique used industrially to multiply plants which are difficult to propagate by conventional means is
1. Protoplast fusion
2. Tissue culture
3.Hybridization
4. Genetic engineering
The technique which is useful in genetic engineering to introduce rDNA and other DNA fragments into higher plant cells in culture is
1. Protoplasmic fusion
2. Tissue culture
3. Hardening
4.Splicing
The transfer of inoculums is done under
1. Aseptic condition
2. Septic condition
3. Hygenic condition
4. Environmental condition
The two different genetic sources protoplasts usually used are
1.Mesophyll and parenchyma cells
2.Mesophyll and epidermal cells
3. Epidermal cells and parenchyma cells
4.Hypodermal and mesophyll cells
Tissue culture technique is successfully employed to bring about
1. Protoplasmic fusion
2. Gene manipulation
3. Splicing
4. Hardening
Vectors are also known as
1.DNA ligase
2.Plasmid
3. Cloning vehicles
4. Inoculum
Very small number of isolated living cells is called
1.Plasmid
2. Vector
3. Auxin
4. Inoculum
Virus free potatoes tulips sugarcanes and orchids are obtained by the technique of
1.Hardening
2. Totipotency
3. Tissue culture
4. Splicing
Yoghurt is a food product produced by employing
1.Hybridization
2.Tissue culture
3.Protoplast fusion
4.Biotechnology