Amino-acids are distinguishable from each other only by one or two atoms in their structure.
1.True
2.False
3.all of the above
4.None of the mentioned
In Automatic amino-acid analyzer, the sample containing ___________ of each amino compound is introduced at the top of the ion exchange column.
1. 1 to 10 µmoles
2.1 to 10 moles
3.0.05 to 2 moles
4.0.05 to 2 µmoles
In which of the following ways can chemiluminescence occur in compounds of nitrogen?
1. When NO2 returns to ground state from excited state
2.When NO reacts with oxygen
3.When NO reacts with ozone
4.When NO2 is reduced to NO
pH meters can be considered as voltage sources with which of the following internal resistances?
1.Very low resistance
2.Moderate resistance
3.Very high resistance
4.ValidationSummary
Propane would give three times the intensity of response as compared to methane.
1.True
2.False
3.all of the above
4.None of the mentioned
Trapped air in pumps of Automatic amino-acid analyzer is eliminated using which of the following ways?
1.Suction pump
2. De-aerator
3. Overcome naturally by pump pressure
4. Using pulse damper
What is the drawback that occurs in using ion exchange chromatography on sulphonated polystyrene resin and colourimetry for amino-acid analysis?
1.Less accuracy
2.Low resolution
3.Inconvenient to handle many individual samples
4. Slow in operation
Which of the following cannot be analysed using resin column chromatography?
1. Peptides
2.Amines
3.Amino compounds
4.Components which are ninhydrin negative
Which of the following is not the characteristic of chopper amplifier pH meter?
1.Direct voltage from the electrodes is chopped at the main frequency
2.Using choppers for high-input resistance gives rise to spikes of waveforms at the output
3. It leads to stability in DC output of phase-sensitive rectifier
4.Magnitude of surge increases in the glass electrode output
Which of the following is the most abundant hydrocarbon which is emitted into the atmosphere by biological activity?
1.Ethane
2.Methane
3. Poly Aromatic hydrocarbons
4.Pentane
Which of the following is the relation between hydrogen and hydroxyl ion concentration of pure water?
1. Value of hydrogen ion concentration is greater
2.Value of hydroxyl ion concentration is greater
3.They are both always the same
4.The concentrations keep changing
CO laser is operated at liquid nitrogen temperature.
1.True
2.False
3.all of the above
4.None of the mentioned
Flame ionization detectors are sensitive to which of the following gases?
1.Carbon dioxide
2.Methane
3.Carbon monoxide
4.Sulphur dioxide
How can carbon monoxide be detected using flame ionization detectors while using gas chromatography technique?
1. By converting it into carbon dioxide
2. By converting it into methane
3.By mixing it with combustible gases
4.It cannot be detected
Hydrogen electrode which is the reference electrode can be used as which of the following?
1.Anode only
2.Cathode only
3. Anode or Cathode
4.Salt bridge
In flame ionization detector, the ions collected on the negatively charged grid are amplified at the high impedance amplifier.
1.True
2.False
3.all of the above
4.None of the mentioned
In flame ionization detectors, which of the following is the range adequate for atmospheric sampling?
1.0-1 ppm
2.0-2 ppm
3.0-20 ppm
4. 0-100 ppm
In Hydrogen electrode, the electrode is placed in a solution of ____ M Hcl. Fill in the blank.
1.0.5
2.1
3.2
4.3
In which of the following ways can the disadvantages of chopper amplifier type pH meter be overcome?
1.Using zero corrected DC amplifier
2.Using modern design
3.Using digital design
4.Using vibrating condenser
In which of the following ways can zero drift be reduced in pH meters?
1.Using filter
2.Giving zero adjustment arrangement
3. Keeping the input impedance high
4.Using balanced and differential amplifiers
Pure water is known to be which of the following?
1.Weak electrolyte
2.Strong electrolyte
3.Neither weak nor strong
4.Not an electrolyte
Resin column chromatography does not involve breaking of bonds in amino-acids.
1.True
2. False
3.all of the above
4.None of the mentioned
Slope factor is independent of temperature.
1.True
2. False
3.all of the above
4.None of the mentioned
The chemiluminescence reactor with ozonator is specific to which of the following compounds?
1.N2
2.NO
3.NO2
4. N2O
The electrodes used in pH measurement have which of the following internal resistances?
1. Very low resistance
2.Moderate resistance
3.Very high resistance
4.No resistance
The measurement of hydrogen ion concentration can be made by measuring the potential developed in an electrochemical cell.
1.True
2.False
3.all of the above
4.None of the mentioned
The Nernst equation is given by which of the following statements?
1.E=Eo + 2.303 RT/F log CH
2.E=Eo – 2.303 RT/F log CH
3. E=Eo + 2.303 RT×F log CH
4.E=Eo – 2.303 RT×F log CH
West-Gaeke colorimetric method is used to analyse oxides of nitrogen
1.True
2. False
3.all of the above
4.None of the mentioned
What is the drawback that occurs in using gas chromatography for amino-acid analysis?
1.Costly
2.Cannot be used for proteins or polypeptides
3.Slow process
4. Complex in operation
Which of the following amino-acids is measured at a wavelength of 440nm using photometric systems?
1.Proline
2. Alanine
3.Glutamine
4.Valine
Which of the following can be used to provide automatic temperature compensation?
1.Proper insulation
2.Calibration for different temperatures
3.Thermistor
4.Thermometer
Which of the following colour reagents are used in Resin column chromatography?
1.Marquis reagent
2. Benedict reagent
3.Ninhydrin
4.Nessler’s reagent
Which of the following is not a failure in pH meters?
1.Defective electrodes
2.Defective input circuitry
3.Defective electronic circuitry
4.Defective calibration
Which of the following is not the characteristic of a reference electrode?
1.It must have a known output potential
2.It must have a constant output potential
3.Its output potential is dependent on the composition of the solution
4.It is employed in conjunction with the indicator or working electrode
Which of the following is not the characteristic of direct reading type pH meters?
1. Simple operation
2. Quick to use
3.Continuous indication output
4.It requires balancing process
Which of the following is not the characteristic of flame ionization detector?
1.Good linearity
2.Narrow range of concentration
3.High sensitivity
4.Fast response
Which of the following is not the characteristic of null-detector type pH meter?
1.It can be battery operated
2. It has less accuracy
3. It is easy to maintain
4.Its electronic circuits are simple
Which of the following is not the disadvantage of hydrogen electrode?
1.Platinum can be easily poisoned
2.Presence of oxidising agents alters the potential
3.It gives a salt error
4.H2 gas at 1 atmospheric pressure is difficult to set up and transport
Which of the following is the formula for pH calculation?
1.log10[H+]
2.-log10[H+]
3. log2[H+]
4. -log2[H+]
Which of the following is the relation between the concentration of hydrogen and hydroxyl ions in a basic solution?
1.Value of hydrogen ion concentration is greater
2.Value of hydroxyl ion concentration is greater
3.They are both always the same
4.The concentrations keep changing
Which of the following is the relation between the concentration of hydrogen and hydroxyl ions in an acidic solution?
1. Value of hydrogen ion concentration is greater
2.Value of hydroxyl ion concentration is greater
3.They are both always the same
4. The concentrations keep changing
Which of the following is the simple and most convenient hydrogen electrode?
1.Pascal Hydrogen electrode
2.Bourne Hydrogen electrode
3.Hilderbant Hydrogen electrode
4.West Hydrogen electrode
Which of the following is the simplest of pH meters?
1.Null-detector type pH meter
2.Direct reading type pH meter
3.Digital pH meter
4.Modern pH meter
Which of the following is the value of hydrogen ion concentration of pure water?
1.1×107 moles/litre
2.1×105 moles/litre
3.1×106 moles/litre
4.1×108 moles/litre
Which of the following is the wavelength at which CO laser operates?
1.1.005 µm
2.5.307 µm
3.4.952 µm
4.6.179 µm
Which of the following methods cannot be used for the estimation of hydrocarbons?
1.Mass spectroscopy
2.Chemiluminescence method
3.Ultraviolet absorption
4.Gas chromatography
Which of the following methods make use of pyrolysis while used for detecting methane?
1.Mass spectroscopy
2.Flame ionization detector
3.Ultraviolet absorption
4.Gas chromatography
Which of the following organic compounds will have the highest intensity of response when introduced in a flame ionization detector?
1. Methane
2.Ethane
3. Propane
4.Butane
Which one of the following methods is the most suitable for amino-acid analysis?
1.Gas chromatography
2.Ion exchange chromatography
3. Paper electrophoresis
4.Resin column chromatography
Why is Standard hydrogen electrode called as the primary reference electrode?
1.It has a known output potential
2.It has a constant output potential
3.Its output potential is independent of the composition of the solution
4.Its output potential is zero volts